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020 _a9781786309778
020 _a9781394276639
_q(electronic bk. : oBook)
020 _a139427663X
_q(electronic bk. : oBook)
020 _z9781786309778
024 7 _a10.1002/9781394276639
_2doi
035 _a(OCoLC)1425166238
040 _aDG1
_beng
_erda
_epn
_cDG1
_dOCLCO
041 _aeng
050 4 _aQP623.5.C36
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_x049000
_2bisacsh
072 7 _aSCI
_x045000
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_2bicssc
082 0 4 _a572.8/8
_223/eng/20240306
100 1 _aMasquida, Benoit,
_eauthor.
245 1 0 _aLooking at ribozymes :
_bbiology of catalytic RNA /
_cBenoit Masquida.
264 1 _aLondon, UK :
_bISTE Ltd. ;
_aHoboken, NJ :
_bWiley,
_c©2024.
300 _a1 online resource (192 pages)
336 _atext
_btxt
_2rdacontent.
337 _acomputer
_bc
_2rdamedia.
338 _aonline resource
_bcr
_2rdacarrier.
340 _2rdacc
_0http://rdaregistry.info/termList/RDAColourContent/1003.
490 1 _aBiology series. Nucleic acids set ;
_vvolume 2.
504 _aIncludes bibliographical references and index.
505 0 _aTable of Contents Foreword vii Preface xi Chapter 1 Fundamentals of RNA and Ribozyme Structure 1 1.1 Sequences and secondary structures 2 1.2 RNA folding, tertiary structures and 3D 8 1.2.1 Secondary structures and RNA folding 8 1.2.2 The pseudoknot 12 1.2.3 The loop E 14 1.2.4 The k-turn 16 1.2.5 Tetra-loop receptors 18 1.2.6 The A-minor pattern 20 1.2.7 Comparative analysis of sequences 24 Chapter 2 Ribozymes and the "Central Dogma" of Molecular Biology 29 2.1 The discovery of RNA catalysis and the central dogma of molecular biology 29 2.2 In search of the primordial polymerase 34 Chapter 3 The Discovery of Ribozymes 37 3.1 The discovery of catalysis by autocatalytic introns 38 3.2 The discovery of RNA catalysis of RNase P 42 3.3 The first consequences of these discoveries 46 3.3.1 The ribosome, a long-ignored ribozyme 47 3.3.2 The modified bases 52 3.4 The spliceosome, another ribozyme 55 3.4.1 Nucleolytic ribozymes 56 Chapter 4 Ribozyme Engineering and the RNA World 73 4.1 Classification of ribozymes 75 4.2 Classification of ribozymes according to catalytic mechanism 76 Chapter 5 Structures of Ribozymes 81 5.1 Structures and catalytic mechanisms of ribozymes 81 5.1.1 Hammerhead ribozymes 81 5.1.2 The example of the hairpin ribozyme 87 5.1.3 The example of the glmS ribozyme 89 5.2 An example of catalysis control: lariat-capping ribozyme 91 Chapter 6 Evolution of the Vision of the Catalytic Mechanisms of Ribozymes, the Hammerhead Ribozyme 99 6.1 Chemistry and catalysis: between general acid/base and metal cations 99 6.2 Difficulties in interpreting catalysis data 103 Chapter 7 The Distribution of Ribozymes in Living Organisms and Molecular Adaptations during Evolution 111 7.1 Ubiquitous ribozymes 111 7.2 Selection pressures at work in ribozyme shaping 113 7.3 Ribozymes in cellular processes: from viroids to eukaryotes 117 7.4 Very human ribozymes 123 Conclusion 133 References 135 Index 165
520 _aBehind the neologism "ribozymes" lies a family of fascinating molecules, ribo-enzymes, which have been relatively little studied. These catalytically active RNAs are found in all strata of life, from viruses to the human genome. At the end of the 1970s, the discovery of a catalytic RNA nestled in an intron, followed by another involved in the maturation of transfer RNAs, led to the discovery of new ribozymes and the transition from a strictly "proteocentric" vision, inherited from the dogma of molecular biology, to a more "nucleocentric" one. Since then, a variety of ribozymes have been identified in genomes, where their functions often remain mysterious. Looking at Ribozymes traces the discovery of these molecules and presents a picture of their functional diversity, catalytic mechanisms and distribution within the tree of life.
545 0 _aAbout the Authors Benoît Masquida is director of research at the French National Center for Scientific Research (CNRS) and carries out his research and teaching activities at the University of Strasbourg, France. He is the author of over 60 publications and is one of France’s leading specialists in ribozymes. Fabrice Leclerc is a research fellow at the CNRS and conducts his research at the Paris-Saclay University, France. He has published numerous articles on RNA catalysis in ribozymes and their role in viroids and in the RNA world.
650 0 _aCatalytic RNA.
_0https://id.loc.gov/authorities/subjects/sh89006601.
655 4 _aElectronic books.
700 1 _aLeclerc, Fabrice,
_econtributor.
830 0 _aBiology series. Nucleic acids set ;
_vvolume 2.
856 4 0 _uhttps://onlinelibrary.wiley.com/doi/book/10.1002/9781394276639
_yFull text is available at Wiley Online Library Click here to view
942 _2ddc
_cER