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100 1 _aSubosa, Bemboy Nino F.
_eauthor
245 1 0 _aProduction of peptone from chicken feathers
264 4 _c2016
520 3 _aPeptones are the product of a protein hydrolysis, which serve as the main nutrient source for bacteria in a culture media. This study aimed to provide a locally feasible process for peptone production. Optimization results for Phase 1 showed that a temperature of 90°C, a digestion time of 4 hours, and a ratio of 0.06 g feathers/mL produced a yield of 50.6%. Statistical analysis showed that E. coli growth on the laboratory-produced peptone is significantly greater than the growth realized on commercial peptone and plate count agar. B. cereus growth on laboratory-produced peptone, however, was significantly lower than the growth on commercial peptone and plate count agar. Purification, drying, and characterization techniques were integrated into the existing process for Phase 2 to obtain peptones with better commercial quality. The maximum yield obtained was 30 g peptone per 100 g feathers. Freeze-dried powders from the purified hydrolysates had reduced odor and moisture as compared to the vacuum dried peptone. The vacuum-filtered batch also approximated the physical characteristics of the standard peptone hydrolysate. Performance testing for Phase 2 showed increased support for bacterial growth.
650 0 _aCulture media (Biology)
700 1 _aMirando, Jevie S.
_eauthor
700 1 _aVelasquez, Allan Paolo V.
_eauthor
700 1 _aAlamar, Vera Loise Q.
_eauthor
700 1 _aCapinpin, Geordan Gerald L.
_eauthor
700 1 _aSablan, Khrizelle Angelique D.
_eauthor
700 1 _aDe Leon, Rizalinda L.
_eauthor
773 _tPhilippine Engineering Journal
_gvol. 37, no. 1: (June 2016), pages 63-78
942 _2ddc
_cART