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100 | 1 |
_aSubosa, Bemboy Nino F. _eauthor |
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245 | 1 | 0 | _aProduction of peptone from chicken feathers |
264 | 4 | _c2016 | |
520 | 3 | _aPeptones are the product of a protein hydrolysis, which serve as the main nutrient source for bacteria in a culture media. This study aimed to provide a locally feasible process for peptone production. Optimization results for Phase 1 showed that a temperature of 90°C, a digestion time of 4 hours, and a ratio of 0.06 g feathers/mL produced a yield of 50.6%. Statistical analysis showed that E. coli growth on the laboratory-produced peptone is significantly greater than the growth realized on commercial peptone and plate count agar. B. cereus growth on laboratory-produced peptone, however, was significantly lower than the growth on commercial peptone and plate count agar. Purification, drying, and characterization techniques were integrated into the existing process for Phase 2 to obtain peptones with better commercial quality. The maximum yield obtained was 30 g peptone per 100 g feathers. Freeze-dried powders from the purified hydrolysates had reduced odor and moisture as compared to the vacuum dried peptone. The vacuum-filtered batch also approximated the physical characteristics of the standard peptone hydrolysate. Performance testing for Phase 2 showed increased support for bacterial growth. | |
650 | 0 | _aCulture media (Biology) | |
700 | 1 |
_aMirando, Jevie S. _eauthor |
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700 | 1 |
_aVelasquez, Allan Paolo V. _eauthor |
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700 | 1 |
_aAlamar, Vera Loise Q. _eauthor |
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700 | 1 |
_aCapinpin, Geordan Gerald L. _eauthor |
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700 | 1 |
_aSablan, Khrizelle Angelique D. _eauthor |
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700 | 1 |
_aDe Leon, Rizalinda L. _eauthor |
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773 |
_tPhilippine Engineering Journal _gvol. 37, no. 1: (June 2016), pages 63-78 |
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942 |
_2ddc _cART |