TY - BOOK AU - Spackman,Erica TI - Avian influenza virus T2 - Methods in molecular biology, SN - 9781588299390 (hbk. : alk. paper) AV - SF995.6.I6 A955 2008 PY - 2008/// CY - Totowa, NJ PB - Humana Press KW - Influenza A virus KW - genetics KW - Laboratory Manuals KW - immunology KW - Orthomyxoviridae Infections KW - virology KW - Virology KW - methods KW - Avian influenza KW - Research KW - Methodology N1 - Includes bibliographical references and index; A brief introduction to the avian influenza virus / Erica Spackman -- Avian influenza virus sample types, collection, and handling / Mary Lea Killian -- Avian influenza virus RNA extraction from tissue and swab material / Erica Spackman and David L. Suarez -- Type A influenza virus detection and quantitation by real-time RT-PCR / Erica Spackman and David L. Suarez -- Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCR -- Avian influenza virus isolation and propagation in chicken eggs / Peter R. Woolcock -- Hemagglutination-inhibition test for avian influenza virus subtype identification and the detection and quantitation of serum antibodies to the avian influenza virus / Janice C. Pedersen -- Neuraminidase-inhibition assay for the identification of influenza A virus neuraminidase subtype or neuraminidase antibody specificity / Janice C. Pedersen -- Immunohistochemical staining for the detection of the avian influenza virus in tissues / Mary J. Pantin-Jackwood -- Wild bird surveillance for the avian influenza virus / Justin D. Brown and David E. Stallknecht -- Reverse genetics of the avian influenza virus / Chang-Won Lee and David L. Suarez -- Evaluating the cell-mediated immune response of avian species to avian influenza viruses / Darrell R. Kapczynski -- Measurement of avian cytokines with real-time RT-PCR following infection with the avian influenza virus / Darrell R. Kapczynski and Michael H. Kogut ER -