Practical guide to diagnostic parasitology / (Record no. 87111)

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100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Garcia, Lynne Shore,
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245 10 - TITLE STATEMENT
Title Practical guide to diagnostic parasitology /
Statement of responsibility, etc. Lynne S. Garcia.
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Edition statement 3rd edition.
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Place of production, publication, distribution, manufacture Hoboken, NJ :
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Date of production, publication, distribution, manufacture, or copyright notice 2021.
300 ## - PHYSICAL DESCRIPTION
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500 ## - GENERAL NOTE
General note Lynne Shore Garcia is the director of LSG & Associates, a firm providing training, teaching, and consultation services for diagnostic medical parasitology and health care administration. A former manager of the UCLA Clinical Microbiology Laboratory, she is a sought-after speaker (nationally and internationally) and author of hundreds of articles, book chapters, and books including two ASM Press books, Clinical Laboratory Management, Second Edition and Diagnostic Medical Parasitology, Sixth Edition
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references and index.
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note TABLE OF CONTENTS<br/>Preface xix<br/><br/>About the Author xxiii<br/><br/>Section 1 Philosophy and Approach to Diagnostic Parasitology 1<br/><br/>Neglected Tropical Diseases 2<br/><br/>Why Perform Diagnostic Parasitology Testing? 3<br/><br/>Travel 3<br/><br/>Population Movements 3<br/><br/>Control Issues 4<br/><br/>Climate Change 4<br/><br/>Epidemiologic Considerations 4<br/><br/>Compromised Patients; Potential Sex Bias Regarding Infection Susceptibility; Aging 4<br/><br/>Approach to Therapy 5<br/><br/>Who Should Perform Diagnostic Parasitology Testing? 5<br/><br/>Laboratory Personnel 5<br/><br/>Nonlaboratory Personnel 5<br/><br/>Where Should Diagnostic Parasitology Testing Be Performed? 6<br/><br/>Inpatient Setting 6<br/><br/>Outpatient or Referral Setting 6<br/><br/>Decentralized Testing 6<br/><br/>Physician Office Laboratories 6<br/><br/>Over-the-Counter (Home Care) Testing 7<br/><br/>Field Sites 7<br/><br/>What Factors Should Precipitate Testing? 7<br/><br/>Travel and Residence History 7<br/><br/>Immune Status of the Patient 7<br/><br/>Clinical Symptoms 7<br/><br/>Documented Previous Infection 7<br/><br/>Contact with Infected Individuals 8<br/><br/>Potential Outbreak Testing 8<br/><br/>Occupational Testing 8<br/><br/>Therapeutic Failure 8<br/><br/>What Testing Should Be Performed? 8<br/><br/>Routine Tests 8<br/><br/>Special Testing and Reference Laboratories 9<br/><br/>Specialized Referral Test Options—DPDx and Other Sites 9<br/><br/>Other (Nonmicrobiological) Testing 10<br/><br/>What Factors Should Be Considered in Development of Test Menus? 10<br/><br/>Physical Plant 10<br/><br/>Client Base 10<br/><br/>Customer Requirements and Perceived Levels of Service 10<br/><br/>Personnel Availability and Level of Expertise 10<br/><br/>Equipment 10<br/><br/>Budget 11<br/><br/>Risk Management Issues Associated with Stat Testing 11<br/><br/>Primary Amebic Meningoencephalitis 11<br/><br/>Granulomatous Amebic Encephalitis and Amebic Keratitis 14<br/><br/>Request for Blood Films 15<br/><br/>Automated Instrumentation 15<br/><br/>Patient Information 16<br/><br/>Conventional Microscopy 16<br/><br/>Table 1.1 Common features of the neglected tropical diseases 18<br/><br/>Section 2 Parasite Classification and Relevant Body Sites 19<br/><br/>Protozoa (Intestinal) 20<br/><br/>Amebae, Stramenopiles 20<br/><br/>Flagellates 22<br/><br/>Ciliates 22<br/><br/>Apicomplexa (Including Coccidia) 23<br/><br/>Microsporidia (Now Classified with the Fungi) 23<br/><br/>Protozoa (Other Body Sites) 24<br/><br/>Amebae 24<br/><br/>Flagellates 24<br/><br/>Apicomplexa (Including Coccidia) 24<br/><br/>Microsporidia (Now Classified with the Fungi) 25<br/><br/>Protozoa (Blood and Tissue) 26<br/><br/>Apicomplexa (Including Sporozoa) 26<br/><br/>Flagellates 26<br/><br/>Nematodes (Intestinal) 29<br/><br/>Nematodes (Tissue) 29<br/><br/>Nematodes (Blood and Tissue) 30<br/><br/>Cestodes (Intestinal) 31<br/><br/>Cestodes (Tissue) 32<br/><br/>Trematodes (Intestinal) 33<br/><br/>Trematodes (Liver and Lungs) 33<br/><br/>Trematodes (Blood) 35<br/><br/>Pentastomids 35<br/><br/>Acanthocephala 36<br/><br/>Table 2.1 Classification of human parasites 37<br/><br/>Table 2.2 Cosmopolitan distribution of common parasitic infections 39<br/><br/>Table 2.3 Body sites and possible parasites recovered 40<br/><br/>Section 3 Collection Options 43<br/><br/>Safety 44<br/><br/>Collection of Fresh Stool Specimens 44<br/><br/>Collection Method 44<br/><br/>Number of Specimens To Be Collected 45<br/><br/>Collection Times 47<br/><br/>Posttherapy Collection 47<br/><br/>Specimen Type, Stability, and Need for Preservation 47<br/><br/>Preservation of Stool Specimens 48<br/><br/>Overview of Preservatives 48<br/><br/>Formalin 49<br/><br/>Sodium Acetate-Acetic Acid-Formalin (SAF) 50<br/><br/>Schaudinn’s Fluid 51<br/><br/>Schaudinn’s Fluid containing PVA (Mercury Base) 52<br/><br/>Schaudinn’s Fluid containing PVA (Copper Base, Zinc Base) 52<br/><br/>Single-Vial Collection Systems (Other than SAF) 53<br/><br/>Universal Fixative (Total-Fix) 54<br/><br/>Quality Control for Preservatives 54<br/><br/>Procedure Notes for Use of Preservatives (Stool Fixative Collection Vials) 55<br/><br/>Procedure Limitations for Use of Preservatives (Stool Fixative Collection Vials) 55<br/><br/>Collection of Blood 56<br/><br/>Collection and Processing 56<br/><br/>Stat Test Requests and Risk Management Issues 57<br/><br/>Collection of Specimens from Other Body Sites 57<br/><br/>Table 3.1 Fecal specimens for parasites: options for collection and processing 58<br/><br/>Table 3.2 Approaches to stool parasitology: test ordering 61<br/><br/>Table 3.3 Preservatives and procedures commonly used in diagnostic parasitology (stool specimens) 62<br/><br/>Table 3.4 Advantages of thin and thick blood films 64<br/><br/>Table 3.5 Advantages and disadvantages of buffy coat films 64<br/><br/>Table 3.6 Potential problems of using EDTA anticoagulant for the preparation of thin and thick blood films 65<br/><br/>Table 3.7 Body sites and possible parasites recovered (trophozoites, cysts, oocysts, spores, adults, larvae, eggs, amastigotes, and trypomastigotes) 66<br/><br/>Section 4 Specimen Test Options: Routine Diagnostic Methods and Body Sites 69<br/><br/>Ova and Parasite Examination of Stool Specimens 70<br/><br/>Other Diagnostic Methods for Stool Specimens 72<br/><br/>Culture of Larval-Stage Nematodes 72<br/><br/>Estimation of Worm Burdens through Egg Counts 73<br/><br/>Hatching Test for Schistosome Eggs 73<br/><br/>Screening Stool Samples for Recovery of a Tapeworm Scolex 74<br/><br/>Testing of Other Intestinal Tract Specimens 74<br/><br/>Examination for Pinworm 74<br/><br/>Sigmoidoscopy Material 75<br/><br/>Duodenal Drainage Material 76<br/><br/>Duodenal Capsule Technique (Entero-Test) 76<br/><br/>Urogenital Tract Specimens 77<br/><br/>Sputum 77<br/><br/>Aspirates 78<br/><br/>Biopsy Specimens 78<br/><br/>Blood 79<br/><br/>Thin Blood Films 79<br/><br/>Thick Blood Films 80<br/><br/>Blood Staining Methods 80<br/><br/>Buffy Coat Films 80<br/><br/>QBC Microhematocrit Centrifugation Method 80<br/><br/>Knott Concentration 81<br/><br/>Membrane Filtration Technique 81<br/><br/>Culture Methods 81<br/><br/>Animal Inoculation and Xenodiagnosis 82<br/><br/>Antibody and Antigen Detection 82<br/><br/>Antibody Detection 82<br/><br/>Antigen Detection, Nucleic Acid-Based Tests, and Molecular Panels 84<br/><br/>Intradermal Tests 84<br/><br/>UV Autofluorescence 84<br/><br/>Table 4.1 Body sites, procedures and specimens, recommended methods and relevant parasites, and comments 86<br/><br/>Table 4.2 Serologic, antigen, and probe tests used in the diagnosis of parasitic infections 94<br/><br/>Section 5 Specific Test Procedures and Algorithms 95<br/><br/>Microscopy 96<br/><br/>CALIBRATION OF THE MICROSCOPE 96<br/><br/>Ova and Parasite Examination 99<br/><br/>DIRECT WET FECAL SMEAR 99<br/><br/>CONCENTRATION (Sedimentation and Flotation) 104<br/><br/>SEDIMENTATION CONCENTRATION (Formalin-Ethyl Acetate) 105<br/><br/>SEDIMENTATION CONCENTRATION USING THE UNIVERSAL FIXATIVE (Total-Fix) 109<br/><br/>FLOTATION CONCENTRATION (Zinc Sulfate) 110<br/><br/>PERMANENT STAINED SMEAR 114<br/><br/>Stains Used in the Permanent Stained Smear 116<br/><br/>TRICHROME STAIN (Wheatley’s Method) 116<br/><br/>IRON HEMATOXYLIN STAIN (Spencer-Monroe Method) 122<br/><br/>IRON HEMATOXYLIN STAIN (Tompkins-Miller Method) 127<br/><br/>MODIFIED IRON HEMATOXYLIN STAIN (Incorporating the Carbol Fuchsin Step) 128<br/><br/>CHLORAZOL BLACK E STAIN 131<br/><br/>Specialized Stains for Coccidia and Microsporidia 132<br/><br/>KINYOUN’S ACID-FAST STAIN (Cold Method) 132<br/><br/>MODIFIED ZIEHL-NEELSEN ACID-FAST STAIN (Hot Method) 136<br/><br/>CARBOL FUCHSIN NEGATIVE STAIN FOR CRYPTOSPORIDIUM (W. L. Current) 139<br/><br/>RAPID SAFRANIN METHOD FOR CRYPTOSPORIDIUM (D. Baxby) 139<br/><br/>RAPID SAFRANIN METHOD FOR CYCLOSPORA, USING A MICROWAVE OVEN (Govinda Visvesvara) 139<br/><br/>AURAMINE O STAIN FOR APLICOMPLEXA (INCLUDING COCCIDIA) (Thomas Hänscheid) 140<br/><br/>MODIFIED TRICHROME STAIN FOR MICROSPORIDIA (Weber, Green Counterstain) 144<br/><br/>MODIFIED TRICHROME STAIN FOR MICROSPORIDIA (Ryan, Blue Counterstain) 146<br/><br/>MODIFIED TRICHROME STAIN FOR MICROSPORIDIA (Evelyn Kokoskin, Hot Method) 149<br/><br/>Fecal Immunoassays for Intestinal Protozoa 150<br/><br/>ENTAMOEBA HISTOLYTICA 150<br/><br/>CRYPTOSPORIDIUM SPP. 151<br/><br/>GIARDIA LAMBLIA 151<br/><br/>KITS UNDER DEVELOPMENT 151<br/><br/>COMMENTS ON THE PERFORMANCE OF FECAL IMMUNOASSAYS 151<br/><br/>ENZYME IMMUNOASSAYS (Antigen Detection, No Centrifugation Recommended) 152<br/><br/>FLUORESCENCE (Visual Identification of the Organisms, Centrifugation Recommended) 152<br/><br/>LATERAL-FLOW CARTRIDGES (Antigen Detection, No Centrifugation Recommended) 153<br/><br/>Larval Nematode Culture 153<br/><br/>HARADA-MORI FILTER PAPER STRIP CULTURE 153<br/><br/>BAERMANN CONCENTRATION 155<br/><br/>AGAR PLATE CULTURE FOR STRONGYLOIDES STERCORALIS 158<br/><br/>Other Methods for Gastrointestinal Tract Specimens 161<br/><br/>EXAMINATION FOR PINWORM (Cellulose Tape Preparations) 161<br/><br/>SIGMOIDOSCOPY SPECIMENS (Direct Wet Smear) 163<br/><br/>SIGMOIDOSCOPY SPECIMENS (Permanent Stained Smear) 166<br/><br/>DUODENAL ASPIRATES 168<br/><br/>Methods for Urogenital Tract Specimens 171<br/><br/>RECEIPT OF DRY SMEARS 171<br/><br/>DIRECT SALINE MOUNT 171<br/><br/>PERMANENT STAINED SMEAR 174<br/><br/>URINE CONCENTRATION (Centrifugation) 176<br/><br/>URINE CONCENTRATION (Nuclepore Membrane Filter) 179<br/><br/>Preparation of Blood Films 181<br/><br/>THIN BLOOD FILMS 182<br/><br/>THICK BLOOD FILMS 184<br/><br/>COMBINATION THICK-THIN BLOOD FILMS 186<br/><br/>RISK MANAGEMENT ISSUES ASSOCIATED WITH BLOOD FILMS 187<br/><br/>USE OF A REFERENCE LABORATORY FOR PARASITE BLOOD DIAGNOSTIC TESTING 188<br/><br/>BLOOD FILM REPORTING WITH ADDITIONAL REPORT COMMENTS 189<br/><br/>BUFFY COAT BLOOD FILMS 189<br/><br/>Blood Stains 190<br/><br/>STAIN OPTIONS 190<br/><br/>GIEMSA STAIN 190<br/><br/>Blood Concentration 194<br/><br/>BUFFY COAT CONCENTRATION 194<br/><br/>KNOTT CONCENTRATION 195<br/><br/>MEMBRANE FILTRATION CONCENTRATION 197<br/><br/>Algorithm 5.1 Procedure for processing fresh stool for the O&P examination 201<br/><br/>Algorithm 5.2 Procedure for processing liquid specimens for the O&P examination 202<br/><br/>Algorithm 5.3 Procedure for processing preserved stool for the O&P examination—two-vial collection kit 203<br/><br/>Algorithm 5.4 Procedure for processing SAF-preserved stool for the O&P examination 204<br/><br/>Algorithm 5.5 Procedure for the use of Total-Fix (universal fixative, single-vial system) (this fixative contains no mercury, no PVA, and no formalin) 205<br/><br/>ALTERNATE METHOD FOR SMEAR PREPARATION DIRECTLY FROM VIAL 206<br/><br/>Algorithm 5.6 Use of various fixatives and their recommended stains 207<br/><br/>Algorithm 5.7 Ordering algorithm for laboratory examination for intestinal parasites 208<br/><br/>Algorithm 5.8 Procedure for processing blood specimens for examination 209<br/><br/>Table 5.1 Body sites, specimens, and recommended stains 210<br/><br/>Table 5.2 Approaches to stool parasitology: test ordering 214<br/><br/>Table 5.3 Laboratory test reports: notes and optional comments 215<br/><br/>Table 5.4 Parasitemia determined from conventional light microscopy: clinical correlation 216<br/><br/>Section 6 Commonly Asked Questions about Diagnostic Parasitology 217<br/><br/>Stool Parasitology 218<br/><br/>Specimen Collection 218<br/><br/>Specimen Processing 221<br/><br/>Diagnostic Methods 222<br/><br/>Stool Immunoassay Options 227<br/><br/>MOLECULAR TEST PANELS (FDA CLEARED) 229<br/><br/>A. APTIMA Trichomonas vaginalis Assay 230<br/><br/>B. Affirm VPIII Microbial Identification Test 231<br/><br/>C. Cepheid Xpert TV Assay for Trichomonas vaginalis from Men and Women 232<br/><br/>D. BD MAX Enteric Parasite Panel 233<br/><br/>E. BioFire FilmArray Gastrointestinal Panel 234<br/><br/>F. Luminex (Verigene II GI Flex Assay; Includes Parasites) 236<br/><br/>G. Other Pending Molecular Tests 238<br/><br/>Organism Identification 238<br/><br/>Reporting 242<br/><br/>Proficiency Testing 245<br/><br/>Tissues or Fluids 245<br/><br/>Blood 246<br/><br/>Specimen Collection 246<br/><br/>Specimen Processing 248<br/><br/>Diagnostic Methods 249<br/><br/>Organism Identification 254<br/><br/>Reporting 256<br/><br/>Proficiency Testing 257<br/><br/>General Questions 258<br/><br/>Section 7 Parasite Identification 265<br/><br/>Protozoa<br/><br/>Amebae (Intestinal) 266<br/><br/>Entamoeba histolytica 266<br/><br/>Entamoeba histolytica/Entamoeba dispar 270<br/><br/>Comments on Entamoeba moshkovskii and Entamoeba bangladeshi 273<br/><br/>Entamoeba bangladeshi 274<br/><br/>Entamoeba hartmanni 275<br/><br/>Entamoeba coli 278<br/><br/>Entamoeba gingivalis 281<br/><br/>Entamoeba polecki 283<br/><br/>Endolimax nana 284<br/><br/>Iodamoeba bütschlii 287<br/><br/>Blastocystis spp. (formerly Blastocystis hominis) 290<br/><br/>Flagellates (Intestinal) Giardia lamblia (G. duodenalis, G. intestinalis) 295<br/><br/>Dientamoeba fragilis 298<br/><br/>Chilomastix mesnili 302<br/><br/>Pentatrichomonas hominis 304<br/><br/>Enteromonas hominis, Retortamonas intestinalis 307<br/><br/>Ciliates (Intestinal) 310<br/><br/>Balantidium coli 310<br/><br/>Apicomplexa (Intestinal) 315<br/><br/>Cryptosporidium spp. 315<br/><br/>Coccidia (Intestinal) 319<br/><br/>Cyclospora cayetanensis 319<br/><br/>Cystoisospora (formerly Isospora) belli 323<br/><br/>Microsporidia (Intestinal) 326<br/><br/>Enterocytozoon bieneusi 326<br/><br/>Encephalitozoon intestinalis, Encephalitozoon spp. 329<br/><br/>Sporozoa (Blood and Tissue) 334<br/><br/>Plasmodium vivax 334<br/><br/>Plasmodium falciparum 339<br/><br/>Plasmodium malariae 343<br/><br/>Plasmodium ovale wallickeri, Plasmodium ovale curtisi 346<br/><br/>Plasmodium knowlesi 350<br/><br/>Malaria 353<br/><br/>Babesia spp. (Babesia microti, B. duncani, B. divergens, B. venatorum) 355<br/><br/>Toxoplasma gondii 359<br/><br/>Flagellates (Blood and Tissue) 368<br/><br/>Leishmania spp. 368<br/><br/>Trypanosoma brucei gambiense (West), T. brucei rhodesiense (East) 372<br/><br/>Trypanosoma cruzi 376<br/><br/>Amebae (Other Body Sites) 381<br/><br/>Naegleria fowleri 381<br/><br/>Acanthamoeba spp., Balamuthia mandrillaris, Sappinia diploidea 385<br/><br/>Flagellates (Other Body Sites) 389<br/><br/>Trichomonas vaginalis 389<br/><br/>NEMATODES 392<br/><br/>Intestinal 392<br/><br/>Ascaris lumbricoides 392<br/><br/>Trichuris trichiura, Capillaria philippinensis 395<br/><br/>Necator americanus, Ancylostoma duodenale, Ancylostoma ceylanicum (Hookworms); Trichostrongylus spp. 398<br/><br/>Strongyloides stercoralis 401<br/><br/>Enterobius vermicularis 404<br/><br/>Tissue 407<br/><br/>Ancylostoma braziliense, Ancylostoma caninum, Uncinaria stenocephala (Dog and Cat Hookworms) 407<br/><br/>Toxocara canis, Toxocara cati (Dog and Cat Ascarid Worms) 410<br/><br/>Dracunculus medinensis 413<br/><br/>Trichinella spiralis 416<br/><br/>Blood and Tissue 419<br/><br/>Filarial Worms 419<br/><br/>CESTODES 425<br/><br/>Intestinal 425<br/><br/>Taenia saginata 425<br/><br/>Taenia solium 428<br/><br/>Diphyllobothrium latum 432<br/><br/>Hymenolepis (Rodentolepis) nana 435<br/><br/>Hymenolepis diminuta 438<br/><br/>Dipylidium caninum 440<br/><br/>Tissue 443<br/><br/>Echinococcus granulosus, Echinococcus multilocularis, Echinococcus vogeli,<br/><br/>Echinococcus oligarthrus 443<br/><br/>TREMATODES 448<br/><br/>Intestinal 448<br/><br/>Fasciolopsis buski 448<br/><br/>Liver and Lungs 451<br/><br/>Paragonimus westermani, Paragonimus mexicanus, Paragonimus kellicotti 451<br/><br/>Fasciola hepatica 454<br/><br/>Clonorchis (Opisthorchis) sinensis (Chinese liver fluke) 457<br/><br/>Blood 460<br/><br/>Schistosoma spp. (Schistosoma mansoni, S. haematobium, S. japonicum,<br/><br/>S. mekongi, S. malayensis, S. intercalatum) 460<br/><br/>Section 8 Common Problems in Parasite Identification 465<br/><br/>Table 8.1 Entamoeba sp. trophozoites versus macrophages 486<br/><br/>Table 8.2 Entamoeba sp. cysts versus PMNs 486<br/><br/>Table 8.3 Entamoeba histolytica versus Entamoeba coli precysts and cysts 487<br/><br/>Table 8.4 Endolimax nana versus Dientamoeba fragilis 488<br/><br/>Section 9 Identification Aids 489<br/><br/>DIAGNOSTIC CONSIDERATIONS 490<br/><br/>Table 9.1 Rapid diagnostic procedures 490<br/><br/>Table 9.2 Diagnostic characteristics for organisms in wet mounts (direct or concentration sediment) 491<br/><br/>Table 9.3 Diagnostic characteristics for organisms in permanent stained smears (e.g., Wheatley’s trichrome, iron-hematoxylin) 491<br/><br/>Identification Key 9.1 Identification of intestinal amebae (permanent stained smear) 491<br/><br/>Identification Key 9.2 Identification of intestinal flagellates 492<br/><br/>Identification Key 9.3 Identification of helminth eggs 493<br/><br/>Identification Key 9.4 Identification of microfilariae 494<br/><br/>PROTOZOA 495<br/><br/>Table 9.4 Intestinal protozoa: trophozoites of common amebae 495<br/><br/>Table 9.5 Intestinal protozoa: cysts of common amebae 497<br/><br/>Table 9.6 Intestinal protozoa: trophozoites of less common amebae 499<br/><br/>Table 9.7 Intestinal protozoa: cysts of less common amebae 499<br/><br/>Table 9.8 Morphologic criteria used to identify Blastocystis spp. 500<br/><br/>Table 9.9 Intestinal protozoa: trophozoites of flagellates 501<br/><br/>Table 9.10 Intestinal protozoa: cysts of flagellates 502<br/><br/>Table 9.11 Intestinal protozoa: ciliate (Balantidium coli) 503<br/><br/>Table 9.12 Apicomplexa 503<br/><br/>Table 9.13 Microsporidia (related to the Fungi): general information 504<br/><br/>Table 9.14 Microsporidia: recommended diagnostic techniques 505<br/><br/>Table 9.15 Comparison of Naegleria fowleri, Acanthamoeba spp., Balamuthia mandrillaris, and Sappinia diploidea 506<br/><br/>Table 9.16 Characteristics of Trichomonas vaginalis 507<br/><br/>Table 9.17 Key characteristics of intestinal tract and urogenital system protozoa 508<br/><br/>HELMINTHS 512<br/><br/>Table 9.18 Normal life spans of the most common intestinal nematodes 512<br/><br/>Table 9.19 Characteristics of the most common intestinal nematodes 513<br/><br/>Table 9.20 Tissue nematodes 515<br/><br/>Table 9.21 Trichinella spiralis: life cycle stages and clinical conditions 517<br/><br/>Table 9.22 Characteristics of human microfilariae 518<br/><br/>Table 9.23 Characteristics of cestode parasites (intestinal) 519<br/><br/>Table 9.24 Tissue cestodes 521<br/><br/>Table 9.25 Characteristics of intestinal trematodes 523<br/><br/>Table 9.26 Characteristics of liver and lung trematodes 524<br/><br/>Table 9.27 Human paragonimiasis 526<br/><br/>Table 9.28 Characteristics of blood trematodes 527<br/><br/>Table 9.29 Key characteristics of helminths 528<br/><br/>BLOOD PARASITES 532<br/><br/>Table 9.30 Malaria characteristics with fresh blood or EDTA-blood 532<br/><br/>Table 9.31 Potential problems with using EDTA anticoagulant for the preparation of thin and thick blood films 533<br/><br/>Table 9.32 Plasmodia in Giemsa-stained thin blood smears 534<br/><br/>Table 9.33 Relevant issues for handling requests for identification of infectious blood parasites 537<br/><br/>Table 9.34 Features of human leishmanial infections 540<br/><br/>Table 9.35 Characteristics of American trypanosomiasis 541<br/><br/>Table 9.36 Characteristics of East and West African trypanosomiasis 541<br/><br/>Table 9.37 Key characteristics of blood parasites 541<br/><br/>Index 547
520 ## - SUMMARY, ETC.
Summary, etc. "As we move forward into the 21st century, the field of diagnostic medical parasitology continues to see some dramatic changes, including newly recognized pathogens, changing endemicity of familiar pathogens, disease control challenges, geographic and climate changes that support the spread of parasitic disease, new methodologies, expansion of diagnostic testing, and an ongoing review of the approach to and clinical relevance of this type of diagnostic testing on patient care within the managed care environment, as well as the world as a whole. The third edition of the Practical Guide to Diagnostic Parasitology is organized to provide maximum help to the user, particularly from the bench use perspective. New aspects of the field have been addressed in these sections, and many new figures and plates have been added, including extensive color images. Section 1 on the philosophy and approach to diagnostic parasitology has been expanded to include discussions on neglected tropical diseases, the impact of global climate change, population movements, potential outbreak testing, the development of laboratory test menus, and the risk management issues related to "stat" testing. The Section 2 discussion of organism classification and relevant tables has been expanded and updated to provide the user with current information related to changes in nomenclature and overall importance of the various parasite categories to human infection"--
Assigning source Provided by publisher.
588 ## - SOURCE OF DESCRIPTION NOTE
Source of description note Description based on print version record and CIP data provided by publisher; resource not viewed.
650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Parasitic Diseases
General subdivision diagnosis
650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Clinical Laboratory Techniques
655 ## - INDEX TERM--GENRE/FORM
Genre/form data or focus term Electronic books.
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element American Society for Microbiology.
856 ## - ELECTRONIC LOCATION AND ACCESS
Link text Full text is available at Wiley Online Library Click here to view
Uniform Resource Identifier <a href="https://onlinelibrary.wiley.com/doi/book/10.1128/9781683673637">https://onlinelibrary.wiley.com/doi/book/10.1128/9781683673637</a>
906 ## - LOCAL DATA ELEMENT F, LDF (RLIN)
a 7
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c orignew
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e ecip
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme
Koha item type EBOOK
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Permanent Location Current Location Date acquired Source of acquisition Inventory number Full call number Barcode Date last seen Price effective from Koha item type
          COLLEGE LIBRARY COLLEGE LIBRARY 2024-04-24 ALBASA Consortium 53005 616.96075 G1655 2021 CL-53005 2024-04-24 2024-04-24 EBOOK