| 000 -LEADER |
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| fixed length control field |
01588nam a2200169Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20200308073501.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
190927s9999 xx 000 0 und d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
T Ab18 2013 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Preferred name for the person |
Abay-Abay, Ellah Jane C. |
| 245 #0 - TITLE STATEMENT |
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| Title |
Extraction and purification of bromelain from pineapple peelings (Ananas comosus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of Publication |
Cebi City |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Name of Publisher |
CIT-U |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Date of Publication |
2013 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc |
The extraction and purification of Bromelain from pineapple (AnanasComosus) peelings are performed. Specifically, the amount and activity of the extracted bromelain from the peelings of the Smooth Cayenne Philippine pineapple cultivar is determined. Bromelain is separated and purified using Polyethylene Glycol (PEG)-Potassium Phosphate Aqueous Two-Phase System at different pH values 5, 6, 7, & 8 and at different w/w PEG-salt concentrations of 18%-14% and 20%-16%. It is assayed for its enzymatic activity via Casein Digestion Unit (CDU) and Bradford method for the protein quantification of the pure extract. It is found that optimum protein concentration is obtained at pH 7.0 and 20% PEG-16% salt ATP system at about 0.66121878µg/ml. CDU results for the proteolytic activity is maximum at 0.345169151 micromoles of L-Tyrosine equivalent released for pH 7.0 18%-14% ATPs concentration. The protein partition (Kp) of 0.862822796 to 0.904185013, enzymatic partition (Ke) of 0.209390765 to 2.654809839, purification factor (PF) of 0.231579557 to 3.076888848, selectivity(S) of 0.231579557 to 3.076888848, and the yield(Y) of 68.68% to 75.52% are obtained. |
| 526 ## - STUDY PROGRAM INFORMATION NOTE |
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| -- |
000-099 |
| 942 ## - ADDED ENTRY ELEMENTS |
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| Item type |
RESERVED BOOKS |
| Source of classification or shelving scheme |
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